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Avidin


All of our standard and customized products are produced from avidin with the same standard. e-Proteins is able to actively participate in research programs that aim to develop a new avidin derivative for new applications.

Avidin is a 66-KDa glycoprotein synthesized in the oviduct of a hen and deposited in the albumen of its eggs. It binds the water-soluble vitamin biotin (vitamin B7 or H) very tightly – irreversibly – and specifically, with an outstanding dissociation constant of ~10-15 M. The protein, its stability and affinity for biotin, have been extensively investigated and characterized. It folds into a quaternary tetramer structure made of 4 subunits of identical amino acid composition and sequence, each containing 9 lysine residues. read more

Sugar heterogeneity of avidin. Comparison of glycopeptides from ovalbumin and avidin shows that the avidin carbohydrate contains at least three distinct oligosaccharide structural types of similar size as shown above; N-linked glycan at Asn-17 consists of GlcNAc(beta1-2)Man(alpha1-3)[GlcNAc(beta1-4)][Man(alpha1-?)Man(alpha1-6)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some terminal galactose may even be present on some glycans.

Avidin vs Neutralite

The deglycosylation reduces the affinity of avidin for lecitins to undetectable levels (Hilles at al., 1987). read more

Benefit of the neutral isoelectric point read more

Other Links

The Avidin-Biotin System (ABS)

Application introduction

Quality for Parenteral Application

Neutralite Avidin is a chemically & enzymatically modified avidin characterized by a neutral isoelectric point and the absence of surface glycans. read more